Journal: Journal of Oral Biology and Craniofacial Research

Article Title: Auricular reconstruction via 3D bioprinting strategies: An update

doi: 10.1016/j.jobcr.2022.07.014

Figure Lengend Snippet: Schematic illustration of the work-flow for auricular Regeneration by 3D Bioprinting.

Article Snippet: The different types of bioinks, their origin, advantages, and limitations are described in . table ft1 table-wrap mode="anchored" t5 caption a7 Source of Bioink Type of bioink Origin and structure Properties in favour of 3D Bioprinting Limitations Natural Gelatin based Bioink Denatured/Partially hydrolyzed form of collagen protein, extracted from bones, skin, and connective tissues of bovine and marine animals - Thermo-responsive - Viscoelastic - Excellent water solubility, biocompatibility and biodegradability, - Promotes cell adhesion, differentiation, migration, and proliferation - Liquefaction temperature (i.e., sol–gel transition point) is 28 °C (25–30 °C) supporting Laser based and Extrusion based Bioprinting due to ease in post-printing removal - Low mechanical strength (can be enhanced by blending with glutaraldehyde - The weight of unmodified gelatin gel decreases by 50% after around 10 h of incubation and the gel completely dissolves within 24 h Fibrinogen/Fibrin based 34 Fibrillar protein formed from fibrinogen circulating in blood obtained from sources like salmon, bovine, porcine, and human.

Techniques:

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: Auricular reconstruction via 3D bioprinting strategies: An update

doi: 10.1016/j.jobcr.2022.07.014

Figure Lengend Snippet: Description of different types of Bioinks used for 3D Auricular Bioprinting.

Article Snippet: The different types of bioinks, their origin, advantages, and limitations are described in . table ft1 table-wrap mode="anchored" t5 caption a7 Source of Bioink Type of bioink Origin and structure Properties in favour of 3D Bioprinting Limitations Natural Gelatin based Bioink Denatured/Partially hydrolyzed form of collagen protein, extracted from bones, skin, and connective tissues of bovine and marine animals - Thermo-responsive - Viscoelastic - Excellent water solubility, biocompatibility and biodegradability, - Promotes cell adhesion, differentiation, migration, and proliferation - Liquefaction temperature (i.e., sol–gel transition point) is 28 °C (25–30 °C) supporting Laser based and Extrusion based Bioprinting due to ease in post-printing removal - Low mechanical strength (can be enhanced by blending with glutaraldehyde - The weight of unmodified gelatin gel decreases by 50% after around 10 h of incubation and the gel completely dissolves within 24 h Fibrinogen/Fibrin based 34 Fibrillar protein formed from fibrinogen circulating in blood obtained from sources like salmon, bovine, porcine, and human.

Techniques: Solubility, Migration, Incubation, Encapsulation, Viscosity, Concentration Assay, Synthesized, Adsorption, Liposomes, Construct, In Vivo, Shear

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: Auricular reconstruction via 3D bioprinting strategies: An update

doi: 10.1016/j.jobcr.2022.07.014

Figure Lengend Snippet: Studies describing employment of 3D bioprinting in auricular reconstruction.

Article Snippet: The different types of bioinks, their origin, advantages, and limitations are described in . table ft1 table-wrap mode="anchored" t5 caption a7 Source of Bioink Type of bioink Origin and structure Properties in favour of 3D Bioprinting Limitations Natural Gelatin based Bioink Denatured/Partially hydrolyzed form of collagen protein, extracted from bones, skin, and connective tissues of bovine and marine animals - Thermo-responsive - Viscoelastic - Excellent water solubility, biocompatibility and biodegradability, - Promotes cell adhesion, differentiation, migration, and proliferation - Liquefaction temperature (i.e., sol–gel transition point) is 28 °C (25–30 °C) supporting Laser based and Extrusion based Bioprinting due to ease in post-printing removal - Low mechanical strength (can be enhanced by blending with glutaraldehyde - The weight of unmodified gelatin gel decreases by 50% after around 10 h of incubation and the gel completely dissolves within 24 h Fibrinogen/Fibrin based 34 Fibrillar protein formed from fibrinogen circulating in blood obtained from sources like salmon, bovine, porcine, and human.

Techniques: Construct, Shear, In Vitro, Adsorption, In Vivo, Encapsulation, Cell Culture

Journal: Journal of Tissue Engineering

Article Title: Meniscus regeneration by 3D printing technologies: Current advances and future perspectives

doi: 10.1177/20417314211065860

Figure Lengend Snippet: 3D bioprinted scaffolds.

Article Snippet: Lee et al. , Laser Scan of human/sheep Meniscus 3D CAD 3D Bioprinter (Bioplotter, EnvisionTEC). Parameters. print T : 120°C. , PCL Mw 65,000 Da , Tethering of CTGF and TGF-β3 incorporated in PLGA microstrands , Meniscus-like 100 μm MESH: (a) interlaid strands and interconnecting microchannels with 100 μm Ø + circumferentially aligned fibers added (human meniscus scaffold); (b) 300 μm microstrands and 100 mm microchannels (sheep meniscus scaffold) , - Human BM or synovium MSCs (Passage: 2–3) , — , Static culture - Cell recruitment - Fibrocartilage matrix formation , Orthotopic implant (sheep) [− cells] End point: 12 weeks - Mechanical behavior (dynamic compression, pull-out strength, friction coefficient, stress relaxation, tensile test).

Techniques: Pore Size, In Vitro, In Vivo, Software, Activity Assay, Computed Tomography, Dispersion, Construct, Diffusion-based Assay, Mouse Assay, Gene Expression

Journal: Journal of Tissue Engineering

Article Title: Meniscus regeneration by 3D printing technologies: Current advances and future perspectives

doi: 10.1177/20417314211065860

Figure Lengend Snippet: Synthetic 3D printed + 3D bioprinted composite scaffolds.

Article Snippet: Lee et al. , Laser Scan of human/sheep Meniscus 3D CAD 3D Bioprinter (Bioplotter, EnvisionTEC). Parameters. print T : 120°C. , PCL Mw 65,000 Da , Tethering of CTGF and TGF-β3 incorporated in PLGA microstrands , Meniscus-like 100 μm MESH: (a) interlaid strands and interconnecting microchannels with 100 μm Ø + circumferentially aligned fibers added (human meniscus scaffold); (b) 300 μm microstrands and 100 mm microchannels (sheep meniscus scaffold) , - Human BM or synovium MSCs (Passage: 2–3) , — , Static culture - Cell recruitment - Fibrocartilage matrix formation , Orthotopic implant (sheep) [− cells] End point: 12 weeks - Mechanical behavior (dynamic compression, pull-out strength, friction coefficient, stress relaxation, tensile test).

Techniques: Pore Size, In Vitro, In Vivo, Gene Expression, Mouse Assay, Software, Dispersion, Micro-CT, In Situ, Expressing, Marker, Construct

Journal: Journal of Tissue Engineering

Article Title: Meniscus regeneration by 3D printing technologies: Current advances and future perspectives

doi: 10.1177/20417314211065860

Figure Lengend Snippet: Synthetic 3D printed bare scaffolds.

Article Snippet: Lee et al. , Laser Scan of human/sheep Meniscus 3D CAD 3D Bioprinter (Bioplotter, EnvisionTEC). Parameters. print T : 120°C. , PCL Mw 65,000 Da , Tethering of CTGF and TGF-β3 incorporated in PLGA microstrands , Meniscus-like 100 μm MESH: (a) interlaid strands and interconnecting microchannels with 100 μm Ø + circumferentially aligned fibers added (human meniscus scaffold); (b) 300 μm microstrands and 100 mm microchannels (sheep meniscus scaffold) , - Human BM or synovium MSCs (Passage: 2–3) , — , Static culture - Cell recruitment - Fibrocartilage matrix formation , Orthotopic implant (sheep) [− cells] End point: 12 weeks - Mechanical behavior (dynamic compression, pull-out strength, friction coefficient, stress relaxation, tensile test).

Techniques: Pore Size, In Vitro, In Vivo, Gene Expression, Staining, Activity Assay, Software, Shear, Cytotoxicity Assay

Journal: Journal of Tissue Engineering

Article Title: Meniscus regeneration by 3D printing technologies: Current advances and future perspectives

doi: 10.1177/20417314211065860

Figure Lengend Snippet: Synthetic 3D printed conditioned scaffolds.

Article Snippet: Lee et al. , Laser Scan of human/sheep Meniscus 3D CAD 3D Bioprinter (Bioplotter, EnvisionTEC). Parameters. print T : 120°C. , PCL Mw 65,000 Da , Tethering of CTGF and TGF-β3 incorporated in PLGA microstrands , Meniscus-like 100 μm MESH: (a) interlaid strands and interconnecting microchannels with 100 μm Ø + circumferentially aligned fibers added (human meniscus scaffold); (b) 300 μm microstrands and 100 mm microchannels (sheep meniscus scaffold) , - Human BM or synovium MSCs (Passage: 2–3) , — , Static culture - Cell recruitment - Fibrocartilage matrix formation , Orthotopic implant (sheep) [− cells] End point: 12 weeks - Mechanical behavior (dynamic compression, pull-out strength, friction coefficient, stress relaxation, tensile test).

Techniques: Pore Size, In Vitro, In Vivo, Suspension, Immunostaining, Polymer, In Situ, Derivative Assay, Gene Expression, Micro-CT, Staining, Recombinant, X-ray Diffraction, Mouse Assay, Injection, Magnetic Resonance Imaging, Light Microscopy, Proximity Ligation Assay, Activity Assay

Journal: International Journal of Bioprinting

Article Title: 3D-printed Bioreactors for In Vitro Modeling and Analysis

doi: 10.18063/ijb.v6i4.267

Figure Lengend Snippet: 3D-printed bioreactors used in mammalian cell culture applications for assessing of cell viability, cell encapsulation, cell/tissue models, cell imaging, cell therapy, and organ-on-chip applications.

Article Snippet: SLA , Cellbricks 3D-bioprinter , High resolution , Gelatin and polyethylene glycol , Liver lobule , Bioreactor for characterization of liver organoid under static conditions , Human hepatoma cell line, human stellate cells , [ ] .

Techniques: Cell Culture, Imaging, Construct, Biomarker Assay, Microscopy, Infection